Surface Plasmon Resonance Monitoring of Mono-Rhamnolipid Interaction with Phospholipid-Based Liposomes

The interactions of mono-rhamnolipids (mono-RLs) with model membranes were investigated through a biomimetic approach using phospholipid-based liposomes immobilized on gold substrate and also by the multiparametric surface plasmon resonance (MP-SPR) technique. Biotinylated bound onto an SPR chip coated streptavidin layer. resulting MP-SPR signal proved efficient binding liposomes. thickness liposome layer calculated modeling was about 80 nm, which matched average diameter mono-RL to film phospholipid monitored morphological changes assessed signal. We demonstrated capacity technique characterize different steps architecture evolution, i.e., from monolayer single bilayer induced interaction mono-RLs. Further washing treatment Triton X-100 detergent left surface. As possible practical application, our method based membrane coupled measurement be robust sensitive analytical tool for detection mono-RLs limit 2 μg mL–1.